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Prof. Lihua Zhang’s Laboratory (Team 1810): Sensitive Separation & Characterization of Bio-molecules


Prof. Dr. Lihua ZHANG

Address :

457 Zhongshan Road,Dalian 116023, China









      The group is led by Prof. Dr. Lihua ZHANG. Since its foundation in 1999, this group has been undergoing rapid development. Now it consists of 26 scientists (including Prof. Yukui ZHANG, Prof. Weibing ZHANG, associate Prof. Dr. Cao YAN, associate Prof. Yang TIAN and Maolan XU, a technician Ms. Xiaodan ZHANG, guest scientists Prof. Prof. Lee CHAO, Prof. Yushu HUO, Prof. Bingqian SHEN, Prof. Jiuyang ZHAO, Prof. Liming WANG ) and 14 graduate students engaging in the development of high efficient separation and high sensitive detection methods for biomolecule, including high performance liquid chromatography (HPLC), capillary electrophoresis (CE), microfluidic chips and their hyphenation with mass spectrometry (MS). In addition, projects on genetic engineering and Chinese traditional medicine have also been undertaken. Up till now, over 100 papers and four books have been published, and more than 10 patents have been applied or entitled.

Research Interests:
      1. High efficient separation and high sensitive detection methods for the analysis of complex samples.
      2. Gene expression, cell incubation, separation and purification of human kallikrein.
      3. Development of HPLC instrument

Research Projects:
      1. Expression and purification of human renal tissue kallikrein from recombinant baculovirus-infected cells (Innovation Program of Dalian Institute of Chemical Physics, 2000-2002, RMB 600,000)
      2. Development of high performance liquid chromatography instruments and their fittings (Mega-projects of Scientific Research, 2001-2003, RMB 2,000,000)
      3. 100 Talent Project (Chinese Acadmey of Sciences, 2001-2003, RMB 2,000,000)
      4. CE instrument for high accuracy quantitative analysis (Supporting Project from Shanghai, 2001-2003, YMB 1,250,000)
      5. Development of high efficiency and high throughput techniques for proteome study of lung cancer (973 state key fundamental research project, 2002-, RMB 360,000)
      6. Study on monolithic columns of capillary electrochromatography with organically functionalized network materials as stationary phase (National Natural Science Foundation, 2002-2004, RMB 185,000)
      7. Real-time multivariate gradient optimization and fast identification of initial conditions in HPLC (National Natural Science Foundation, 2002-2004, RMB 180,000)
      8. Research on tri-function pressurized CEC (Science and Technology Project from Pudong, Shanghai, 2002-2004, RMB 300,000)
      9. Construction of cDNA phage display library of hepatoma cells (Innovation Program of Dalian Institute of Chemical Physics, 2003-2005, RMB 1,300,000)
      10. Automated tri-functional pressurized CEC (Innovation project from Shanghai, 2003-2005, RMB 1,500,000)

  • Prof. A. Ketttrup, GSF-National Environment and Health Research Center, Germany;
  • Prof. Lee Chao, Medical Univ. of South Carolina, US
  • Prof. Yushu Huo, The University of Texas, US
  • Prof. Yoshinobu Baba, The University of Tokushima, Japan
  • Prof. Zhenfeng Zheng, National Chueng Hsing University, Taiwan

          In addition, Prof. Yukui Zhang in charge of the organization of separation science symposium with Germany and Japan, as well as Beijing Conference and Exhibition on Instrumental Analysis.

  • Shanghai Institute for Biological Sciences
  • Beijing Institute of Chemistry
  • Changchun Institute of Applied Chemistry (Chinese Academy of Sciences), Beijing
  • University, Nanjing University
  • Zhejiang University
  • Fudan University
  • Nankai University
  • Dalian University of Technology